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Ion Exchange Column
 
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This video demonstrates the use of a cation exchange column to convert a salt to an acid. This allows for the analysis of the total number of cations present in a given salt sample by titrating the resulting acid with a base
Views: 78485 crharrison
Ion Exchange Chromatography
 
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Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/ion-exchange-chromatography Facebook link: https://www.facebook.com/aklectures Website link: http://www.aklectures.com
Views: 157975 AK LECTURES
Ion Exchange Chromatography | Principle, Instrumentation & Lab Experiment
 
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This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram This lecture explains about the instrumentation, principle of Ion exchange Chromatography. Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column—a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. Article source: wikipedia Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! Subscribe: https://www.youtube.com/channel/UCU91ilky-nR0VwKYFmJTsUA?sub_confirmation=1 HPLC Chromatography: https://youtu.be/9P_SIiMheZk UV Visible Spectroscopy: https://youtu.be/1mrUHrZ-0zk #BaaYo #Chromatography
Views: 50460 BaaYo
Principles of Ion Exchange Chromatography
 
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Handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Ion Exchange Chromatographic Column: Ion Separation
 
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Please visit our website at http://en.sheng-han.com Qingdao Shenghan Chromatography Technology Co., Ltd. is China's largest manufacturer of ion chromatography. The company specializes in the R & D, production, sales and technical services of the ion chromatograph and related accessories, and is focused on providing global users with leading ion chromatography solutions and technical services.
Views: 12 Sheng Han
Ion Exchange Chromatography - Theory and Principle
 
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http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 166513 Bio-Resource
Ion Exchange Column
 
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Ion Exchange Column
Views: 20657 BCHKID1
DEAE Sephacel Column:  Protein Purification via Ion Exchange
 
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Introduction to Ion Exchange Chromatography for Protein Purification.
Views: 10907 acr92651
Ion exchange resin columns
 
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Views: 8228 RD L
IonExchange
 
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How ion exchange can be used to soften hard water.
Views: 162216 Clayton Spencer
ION EXCHANGE COLUMN
 
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Views: 8553 symecengineers
Ion Exchange Process For Water Softening
 
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On this channel you can get education and knowledge for general issues and topics
Views: 154017 LEARN AND GROW
Quick guide to performing ion exchange chromatography
 
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An breif introduction to cation exchange columns. 1. Draining the equilibration buffer. 0m0s 2. Load and run the Amino Acid (AA) mixture. 1m37s 2. Load Citrate buffer to run AA mixture further into column. 4m24s 3. Summary diagram? 6m56s Note the video was recorded during the class and includes a practical demonstration and a written explanation (pen and paper). These two discussions were spliced together and each cover the same material.
Views: 26750 Mark Temple
Anion Exchange Chromatography - separating haemoglobin and catalase
 
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This video demonstrates how to pour a DEAE-Sepharose column for the separation of the proteins haemoglobin and catalase. The video also shows the running of the column and the separation of the proteins.
Views: 17251 Nick Morris
Introduction to Ion-exchange chromatography
 
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This video explains the fundamentals of ion exchange chromatography and demonstrates buffer selection for protein analysis. More information: https://www.agilent.com/en/products/liquid-chromatography/infinitylab-lc-workflow-solutions/infinitylab-bio-inert-lc-solutions/1260-infinity-ii-bio-inert-lc-system
Views: 69199 Agilent Technologies
The Principle Of Ion Exchange Chromatography, A Full Explanation
 
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This video is an explanation of column chromatography, we will speak about ion exchange chromatography, its princple and how to perform it
Backwashing an Ion Exchange Column
 
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This video shows how to backwash an ion exchange column prior to regenerating the column.
Views: 2103 UAlbertaScience
Metrohm chemical Suppressor for Ion Chromatography
 
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Metrohm Chemical suppressor
Views: 18497 automaion
Ion Exchange Process
 
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This animation shows the process that happens during ion exchange
Views: 24082 Alonso Rocha Tapia
Ion exchange chromatography in Hindi
 
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Ion exchange chromatography in hindi - This lecture explains about the ion exchange chromatography in hindi. Ion exchange chromatography is used to separate proteins based on the or charge. Cation exchange chromatography as well as anion exchange chromatography are used to separate proteins from mixtures. Stay tuned to know more about the following - 1. What is ion exchange chromatography 2. Ion exchange chromatography principle 3. Ion exchange chromatography steps 4. Ion exchange chromatography mechanism. 5. Applications of ion exchange chromatography 6. Types of ion exchange chromatography 7. Difference between cation exchange chromatography and anion exchange chromatography For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the video lecture on ion exchange chromatography in hindi.
Views: 3313 Shomu's Biology
Ion exchange chromatography | cation exchange chromatography and anion exchange chromatography
 
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This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 205056 Shomu's Biology
Packing a Chromatography Column
 
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Chromatography is a biotechnology technique that allows us to separate molecules by size, shape, charge, or other physical properties. In this video, you will learn how to pack a column used for chromatography. http://www.edvotek.com/108 http://www.edvotek.com/243 http://www.edvotek.com/255 http://www.edvotek.com/277 http://www.edvotek.com/302? © 2015 Edvotek Inc. All rights reserved. www.edvotek.com
Views: 4962 Edvotek Inc.
Ion Chromatography
 
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Views: 42419 Bob Burk
An Introduction to Ion Chromatography
 
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Watch this entertaining and educational video to learn how ion chromatography is used to separate different ions in a mixture. Understand the difference between the stationary phase and mobile phase, the roles of different types of columns and detectors. Also hear about the benefits of automated eluent generation and consumables tracking for consistent, accurate results. At the end, we’ll introduce you to our newest system, the Thermo Scientific™ Dionex™ Integrion™ HPIC™ system, a high-pressure ion chromatography system that incorporates all of these important features.
Ion Exchange Column - Fixed Bed
 
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To learn more about ion exchange columns, visit the Visual Encyclopedia of Chemical Engineering: http://encyclopedia.che.engin.umich.edu/Pages/SeparationsChemical/IonExchangeColumns/IonExchangeColumns.html
HILIC Columns Tips & Tricks: Understand the Role of Ion Pairing
 
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Consider how ion pairing shields proteinaceous residues from interacting with the amide stationary phase in large molecule HILIC separations.
Views: 404 Waters Corporation
Ion Exchange Chromatography
 
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Views: 21187 Aurele Ben
Ion Exchange Column - Ion Exchange
 
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To learn more about ion exchange columns, visit the Visual Encyclopedia of Chemical Engineering: http://encyclopedia.che.engin.umich.edu/Pages/SeparationsChemical/IonExchangeColumns/IonExchangeColumns.html
Basic for Operational and Column Design of Ion Exchange
 
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Its a basic understanding of operational and column design of ion exchange column... Enjoy the info... #credit to yana, Ain n zatul
Views: 270 Solihin Hamzah
ION EXCHANGE CHROMATOGRAPHY
 
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IT IS A PROCESS USED TO SEPERATE POLAR MOLECULES FROM A MIXTURE OF MOLECULES. AFFINITY CHROMATOGRAPHY AThttps://youtu.be/JOciAGFfqyA BASICS OF CHROMATOGRAPHY AT https://youtu.be/zsI69-V0biw
Views: 11371 Chalk Talk Tutorials
Anion Exchange Chromatography (GFP Purification part 2 of 6)
 
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The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
Views: 18620 BioNetwork
Ion Exchange
 
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A video demonstrating the CHEM 1002 experiment on ion exchange.
Views: 20572 Bob Burk
Cation Exchange Chromatography
 
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Website: simunyubiomedvisuals.com
Views: 32653 Faith Simunyu
Unused- Graver Water Systems, Cation Vessel Ion Exchange Column Tank - stk#46532007
 
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Unused- Graver Water Systems Approximate 4700 Gallon Cation Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 316/316L stainless steel, vertical. 120" Diameter x 76" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 4" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 4" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7985, serial# 9261-07A, built 2009.
Ion Exchange Chromatography Column Elution
 
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The following is a lecture given by Damien Terry at Texas A&M University for Biochemistry II Lab. It is a guide to column elution in ion exchange chromatography.
Ion Chromatography: UBC CHEM 3rd year Analytical Chemistry
 
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Metrohm IC, ion chromatography, anion, column, conductivity, detector, aqueous, UBC Chemistry
Views: 16319 Robin Stoodley
Ion-exchange chromatography
 
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Analytical Technologies in Biotechnology by Dr. Ashwani K Sharma,Department of Biotechnology,IIT Roorkee.For more details on NPTEL visit http://nptel.iitm.ac.in
Views: 3587 nptelhrd
022-Ion Exchange Chromatography
 
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Discussion of principles behind ion exchange chromatography
Ion Exchange chromatography, How to detect compound  that elutes from your column
 
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While washing of my compound from dowex resin, energy is released. This energy is coming from product released from the resin and can be felt and even measured as an elevation in temperature. In this case its even 25 degrees Celsius.
Views: 830 BoltYes
Ion exchange chromatography
 
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This chromatography lecture explains about ion exchange chromatography.Web- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html This video tutorial explains the mechanisms of ion exchange chromatography and its use in biochemistry
Views: 69559 Shomu's Biology
Ion Exchange Resins, Cation Exchange Resins, Anion Exchange Resins
 
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ION EXCHANGE RESINS Ion exchange resins are cross lined polymer which contains some ions (Cations or Anions) which can be exchanged by the medium ions.
Views: 26231 vedupro
Webinar: Tips for successful ion exchange chromatography
 
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www.gelifesciences.com/ProteinResearch Watch this 50-minute webinar and learn how to: • Optimize the resolution/purity of your target protein • Maximize protein recovery • Reduce hands-on time by optimizing your system’s configuration • Select the right ion exchange chromatography columns and resins to boost your results and efficiency
Unused- Graver Water Systems, Mixed Bed Vessel Ion Exchange Column Tank - stk# 46532002
 
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Unused- Graver Water Systems Approximate 4000 Gallon Mixed Bed Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 304/304L stainless steel, vertical. 120" Diameter x 60" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 3" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 3" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7984, serial# 9261-06E, built 2009.
Column chromatography in Hindi
 
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Column chromatography in hindi - This lecture explains about the principle of column chromatography in details. This video lecture explains the different types of column chromatography and also about the column chromatography basics, steps of column chromatography and how the comun chromatography is performed. Stay tuned to know more about the types of column chromatography like - Affinity chromatography Ion exchange chromatography Gas chromatography Hplc chromatography In column chromatography, two different phases are used to separate molecules based on their properties like charge, size, mass and polar nature. Know more about the column chromatography in hindi. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the lecture on column chromatography in hindi.
Views: 2977 Shomu's Biology
Unused- Graver Water Systems, Mixed Bed Vessel Ion Exchange Column Tank - stk# 46532001
 
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Unused- Graver Water Systems Approximate 4000 Gallon Mixed Bed Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 304/304L stainless steel, vertical. 120" Diameter x 60" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 3" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 3" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7983, serial# 9261-06D, built 2009.